New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seqmmi_7026 1..13

New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq

Recent advances in high-throughput sequencing present a new opportunity to deeply probe an organism's transcriptome. In this study, we used Illumina-based massively parallel sequencing to gain new insight into the transcriptome (RNA-Seq) of the human malaria parasite, Plasmodium falciparum. Using data collected at seven time points during the intraerythrocytic developmental cycle, we (i) detect...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Validation of microscopic diagnosis of malaria in field laboratories of malarious areas of Iran by Nested PCR

Introduction: Examination of Geimsa stained blood smears is the main method of malaria diagnosis in our country, however, accuracy of results is largely depended on the skill and laboratory conditions. This study was designed and carried out to assess the current situation and to determine the effectiveness and quality improvement of malaria diagnosis program. Methods: In this analytical s...

Characterization of the Small Exported Plasmodium falciparum Membrane Protein SEMP1

Survival and virulence of the human malaria parasite Plasmodium falciparum during the blood stage of infection critically depend on extensive host cell refurbishments mediated through export of numerous parasite proteins into the host cell. The parasite-derived membranous structures called Maurer's clefts (MC) play an important role in protein trafficking from the parasite to the red blood cell...

A Comparative in vitro Study of the Effect of Eosin B on Asexual Blood Stages and Gametocyte of Plasmodiun falciparum

Background and Objective: Malaria is one of the most life-threatening infectious diseases worldwide. Transmission of the parasite from human to vector mosquitoes is carried out by the gametocyte of the Plasmodium parasite, while these cells are not involved in the symptoms of the disease. The control of the human to mosquito  transmission stage of the parasite life cycle by antigametocyte drugs...